Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria

810 Field evaluation of a malaria rapid diagnostic test ( ICT Pf )

Malaria can be diagnosed using several approaches including clinical presumptive diagnosis, microscopy, polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA) and malaria rapid diagnostic tests (MRDTs),1 of which microscopy remains the ‘gold standard’.2 Since microscopy requires highly skilled staff, electricity, microscopes and laboratory reagents, its use is restricted to ...

A comparison of antigen dipstick assays with polymerase chain reaction (PCR) technique and blood film examination in the rapid diagnosis of malaria.

Early diagnosis of malaria for military personnel training in the field is crucial in providing proper treatment for the infected and in taking appropriate preventive measures for the non-infected. Present preliminary diagnosis of malaria in the field depends on the clinical symptoms of the patients and there is a need for rapid diagnosis of malaria in the field. The presence of drug-resistant ...

EVALUATION OF DIRECT MICROSCOPY, CULTURE, AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF TUBERCULOUS MENINGITIS

In view of the importance of early diagnosis of tuberculous meningitis (TBM), the efficiency of the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was compared with conventional methods (acid-fast microscopy and culture) for the detection of M. tuberculosis in cerebrospinal fluid (CSF) specimens from patients suspected of TBM. Of the 29 CSF specim...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Comparison of Polymerase Chain Reaction, Ziehl-Neelsen Staining and Histopathologic Findings in Formalin-fixed, Paraffin-Embedded Tissue Specimens for Diagnosis of Tuberculosis

Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study was to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of ...